This study was design to assess Moringa oleifera, seed powder as bio-coagulant in wastewater and groundwater treatment. Fresh, healthy and matured seeds of Moringa oleifera, were bought from Ekeonunwa market Owerri and processed into fine powders. Qualitative phytochemicals screening of aqueous extracts of the seed powders were carried out. Physicochemical parameters such as colour, odour, appearance was checked using ten (10) different observers while pH, turbidity, electrical conductivity (EC), total dissolved solids (TDS), biological oxygen demand (BOD), dissolved oxygen (DO) and chloride (Cl-) were assessed according to standard technique. The result revealed the presence of phytochemicals; alkaloids, flavonoid, phenols, tannins, steroids, saponins, and anthraquinonesin M. oleifera, the treatment of groundwater indicates a change in colour, odour, appearance, and pH, which compare favorably with the control while temperature, EC, TDS, DO, BOD and Cl- were statistically the same before and after treatment,while the turbidity increases across the different treatment.